Differential Stain: Endospore Stain: Schaeffer-Fulton Method

The Schaeffer-Fulton stain is a technique designed to isolate endospores by staining any present endospores green, and any other bacterial bodies red.[1] The green stain is malachite green, and the counterstain is safranin, which dyes any other bacterial bodies red.
source: http://en.wikipedia.org/wiki/Schaeffer-Fulton_stain
BACILLUS SUBTILIS
source: http://archive.microbelibrary.org/asmonly/details.asp?id=2565




OTHER VIDEOS RELATED TO ENDOSPORE STAINING





VIDEO ON ENDOSPORE


An endospore is a dormant, tough, and temporarily non-reproductive structure produced by certain bacteria from the Firmicute phylum.[1] The name "endospore" is suggestive of a spore or seedlike form (endo means within), but it is not a true spore (i.e. not an offspring). It is a stripped-down, dormant form to which the bacterium can reduce itself. Endospore formation is usually triggered by a lack of nutrients. In endospore formation, the bacterium divides within its cell wall. One side then engulfs the other. Endospores enable bacteria to lay dormant for hundreds of years or longer. Revival of spores millions of years old has been claimed.[2] When the environment becomes more favorable, the endospore can reactivate itself to the vegetative state. Most types of bacteria cannot change to the endospore form, but examples include Bacillus and Clostridium.[3]
The endospore consists of the bacterium's DNA and part of its cytoplasm, surrounded by a very tough outer coating.
Endospores can survive without nutrients. They are resistant to ultraviolet radiation, desiccation, high temperature, extreme freezing and chemical disinfectants. Common anti-bacterial agents that work by destroying vegetative cell walls don't work on endospores. Endospores are commonly found in soil and water, where they may survive for long periods of time.
Some classes of bacteria can turn into exospores, also known as microbial cysts, instead of endospores. Exospores and endospores are two kinds of "hibernating" or dormant stages seen in some classes of microorganisms.
source: http://en.wikipedia.org/wiki/Endospore

source: http://lecturer.ukdw.ac.id/dhira/BacterialStructure/Inclusions.html
BACILLLUS SUBTILIS. source: http://noticiadeportiva.com/26.php?q=endospores-bacillus-subtilis&page=5
source: http://pscantie.myweb.uga.edu/definition.html
MNEMONIC FOR THE STRUCTURE OF THE BACTERIA ENDOSPORE:

EXCUSE ME --> EXOSPORIUM
SIR ---> SPORE COAT
ONE ---> OUTERMEMBRANE
CUP ---> CORTEX
IN ----> INTERMEMBRANE
STADIUM ----> SPORE CELL

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